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Role of histidines in the binding of violaxanthin de-epoxidase to the thylakoid membrane as studied by site-directed mutagenesis

Regulation of violaxanthin de-epoxidase (VDE) involves a conformational change at low lumenal pH, followed by binding of the enzyme to the thylakoid membrane. The role of histidine residues in this process was studied by release of unbound enzyme from thylakoids upon sonication, on a pH scale from 4.7 to 7.1. The co-operativity for binding of spinach VDE (four histidines) to the membrane was found

Non-invasive detection of c-myc p64, c-myc p67 and c-erbb-2 in colorectal cancer

Objective. Colorectal cancer is a major cause of cancer mortality in the industrialized nations in the West. Because mortality is closely related to the stage of the disease at the time of diagnosis, detection at an early stage is likely to result in improved recovery rates. Since current diagnostic procedures such as colonoscopy are invasive and the fecal occult blood test (FOBT) lacks sensitivit

Search for SUSY in the AMSB scenario with the DELPHI detector

The DELPHI experiment at the LEP e (+) e(-) collider collected almost 700 pb(-1) at centre-of-mass energies above the Z(0) mass pole and up to 208 GeV. Those data were used to search for SUSY in the Anomaly Mediated SUSY Breaking (AMSB) scenario with a flavour independent common sfermion mass parameter. The searches covered several possible signatures experimentally accessible at LEP, with either

Prospective study of transitional cell carcinoma in the prostatic urethra, and prostate in cystoprostatectomy specimen

Objectives. To prospectively evaluate the incidence of transitional cell carcinoma (TCC) in the prostatic urethra and prostate in the cystoprostatectomy specimen, investigate characteristics of bladder tumours in relation to the risk of involvement of the prostatic urethra and prostate and examine the sensitivity of preoperative loop biopsies from the prostatic urethra. Material and methods. Preop

Flödesanalys i den hållbara utvecklingens tjänst - Reflektioner kring en "metabolism"-studie av Rhenområdets utveckling

The objective of this study is to contribute to the discussion about linking society and nature in environmental research. It is an attempt to provide a critical analysis of the "Industrial Metabolism" studies, which have been based on a flow perspective and have argued that such a perspective is essential to the search for environmentally sustainable societal development paths and could serve as

Characterization of dilatation induced by electrical field stimulation in mammalian cerebral and peripheral vessels

The ability of electrical field stimulation in releasing transmitter from isolated blood vessels in vitro, during recordings of constrictor or dilator responses, is dependent upon an appropriate choice of stimulation parameters which avoid concomitant change in tone due to a direct effect on the vascular smooth muscle membrane. In many species, including man, small arteries such as pial arteries f

Measurements of the stopping forces for heavy ions in Ge, Ag and Au using novel 'polka-dot' detectors

Measurements of the stopping forces for C-14, N-14 and O-16 ions in Ge and Au, for N-14 and F-19 ions in Ag, as well as for F-19 ions in Au have been made, respectively. A novel technique, reported recently, using PIN diodes coated directly with the stopping medium in a polka dot pattern was used. This provided a set of precise, self-consistent measurements on the same stopping medium. Results sho

To be or not to be, unraveling molecular mechanisms for lineage decisions in developing blood cells

All hematopoietic cells originate from hematopoietic stem cells (HSC) residing in the BM. The process of differentiation, through which HSCs generate progenitors and subsequently mature blood cells has been extensively studied but many of the regulatory mechanisms involved remains elusive. The classical model for hematopoiesis has been established based on the identification of common myeloid prec