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Cartilage induction by controlled mechanical stimulation in vivo

To study mechanical control of tissue differentiation, we designed a new version of the previously described bone conduction chamber. The bone conduction chamber consists of a cylindrical titanium chamber for implantation in the rat tibia. It has tissue ingrowth openings at one end, located subcortically, and the other end protrudes into the subcutis. The newly developed load chamber has a mobile

Elevated atmospheric CO2 concentration and diurnal cycle induce changes in lipid composition in Arabidopsis thaliana

Few studies regarding the effects of elevated atmospheric CO2 concentrations on plant lipid metabolism have been carried out. Here, the effects of elevated CO2 concentration on lipid composition in mature seeds and in leaves during the diurnal cycle of Arabidopsis thaliana were investigated. Plants were grown in controlled climate chambers at elevated (800 ppm) and ambient CO2 concentrations. Lipi

Naturally occurring short splice variant of CYLD positively regulates dendritic cell function

Deubiquitination of NF-kappa B members by CYLD is crucial in controlling the magnitude and nature of cell activation. The role of the naturally occurring CYLD splice variant in dendritic cell (DC) function was analyzed using CYLDex7/8 mice, which lack the full-length CYLD (flCYLD) transcript and overexpress the short splice variant (sCYLD). Bone marrow-derived DCs from CYLDex7/8 mice display a hyp

Combining magnets and dielectrics: Crystal chemistry in the BaO-Fe2O3-TiO2 System.

Phases observed in the BaO-Fe2O3-TiO2 system are classified and described in terms of the framework of close-packed {O,Ba/O} layers, with the transition metal atoms either in octahedral (titanium and iron) or in tetrahedral (iron only) sites. The resulting crystal chemistry features new combinations of structural features intermediate between the magnetic barium hexaferrites and the dielectric bar

On Commodification and the Governance of Academic Research

The new prominence given to science for economic growth and industry comes with an increased policy focus on the promotion of commodification and commercialization of academic science. This paper posits that this increased interest in commodification is a new steering mechanism for governing science. This is achieved by first outlining what is meant by the commodification of scientific knowledge t

ELTs, AO wavelengths and science output

A study is presented of the impact on science data from extremely large telescopes of a transformation of wavelength base for optimization of actuator architecture from 2 200 to 1250 nm. From the optical path difference (OPD) data for Euro50, we transform to E-ELT OPDs. We compute the corresponding power spectrum, in which we simulate a higher actuator density via high-pass filtering to convert fr

Enhanced efficiency of industrial-scale anaerobic digestion by the addition of glycosidic enzymes

The results of the action of two glycosidic enzymes added to an anaerobic digester processing mixed wastewater sludge are reported in this paper. The experiment was performed at a continuously operating full-scale wastewater treatment plant during a 6-month period. The addition of the enzyme mixture resulted in improved gas production and dewatering properties. Dewatering experiments, both on enzy

Monte Carlo simulations of defect-free cross-linked gels in the presence of salt

Properties of polyelectrolyte gels in equilibrium with a reservoir of salt solution described within the primitive model have been investigated using Monte Carlo simulations. The polyelectrolyte gel was modeled as a charged defect-free three-dimensional network of diamondlike topology with explicit simple ions. The deswelling upon addition of salt was investigated for gels with different charge de

MRI thermometry in phantoms by use of the proton resonance frequency shift method: application to interstitial laser thermotherapy

In this work the temperature dependence of the proton resonance frequency was assessed in agarose gel with a high melting temperature (95 degrees C) and in porcine liver in vitro at temperatures relevant to thermotherapy (25-80 degrees C). Furthermore, an optically tissue-like agarose gel phantom was developed and evaluated for use in MRI. The phantom was used to visualize temperature distribution

Silanized surfaces for in vitro studies of actomyosin function and nanotechnology applications

We have previously shown that selective heavy meromyosin (HMM) adsorption to predefined regions of nanostructured polymer resist surfaces may be used to produce a nanostructured in vitro motility assay. However, actomyosin function was of lower quality than on conventional nitrocellulose films. We have therefore studied actomyosin function on differently derivatized glass surfaces with the aim to

A 10-bit 500-MS/s 124-mW subranging folding ADC in 0.13 μm CMOS

A 10-bit two-step subranging folding analog-to-digital converter (ADC) that converts signal at 500 MSample/s is presented. Using dual-channel preprocessing blocks with distributed sample-and-hold circuits and two-stage amplifiers in which auto-zero calibration technique is employed, the proposed 10-bit ADC has a wide input bandwidth (>250MHz). The ADC consumes 124mW from a 1.2V power supply. The p

Parallel gene expression profiling of mantle cell lymphoma - How do we transform 'omics data into clinical practice

DNA microarray technology has been a valuable tool to provide a global view of the changes in gene expression that characterize different types of B cell lymphomas, both in relation to clinical parameters but also in comparison with the non-malignant counterparts. The number of transcripts that can be analyzed on an array has dramatically increased, and now most commercially available arrays cover

Characterization of supermacroporous monolithic polyacrylamide based matrices designed for chromatography of bioparticles

Supermacroporous monolithic acrylamide (AAm)-based cryogels were prepared by radical cryo-polymerizaton (polymerization in the moderately frozen system) of AAm with functional monomers and cross-linker N,N'-methylene-bis-acrylamide (MBAAm). Electron microscopy studies revealed supermacroporous structure of the developed cryogels with pore size of 5-100 mum. Cryogel porosity depended on cryo-polyme

A linear response approach to second-order electronic transition intensities for multiconfigurational self-consistent field wave functions

A new theoretical approach to two-photon transition intensities at the multiconfigurational self-consistent field (MCSCF) level of theory, is described in detail. The fundamental property of an MCSCF wave function, that it is possible to define the response equations for an excited state, is a prerequisite. The method requires solely first-order multiconfigurational response calculations, because