Effect of phosphomimetic point mutations on the ADP-ribosylation activity of PARP16
Introduction: Phosphomimetic residue and D-loop mutants have been created and their effect studied in the enzyme PARP16. Background: It is known that PARP16 is phosphorylated, and where the phosphorylation sites are, but not how this affects ADP-ribosylation activity. As of yet, there are no reliable ways to obtain 100% phosphorylated PARP16, so it is instead mimicked with amino-acids with simil